Constructed wetlands for domestic wastewater treatment in a Mediterranean climate region in Chile

Background: Constructed wetlands are a promising, cheap and effective wastewater treatment in small communities. The studies on these systems have been reported mainly from cold, tropical or subtropical climate regions. In this work we constructed a pilot plant with six horizontal subsurface flow constructed wetlands (HSSF CWs) with a surface area of 2 m² and a depth of 0.6 m each, planted with Typha latifolia or Scirpus sp., and filled with gravel (G) or fine gravel (FG) of 2.8 and 1.2 cm of diameter respectively, continuously fed with raw domestic wastewater. This experimental setup was evaluated over 280 days for the removal of organic matter and nutrients in a Mediterranean climate, near Valparaíso, Chile. The removal of total COD, NH4+-N and PO4-3-P was calculated, in order to assess by analysis of variance the effect of initial pollutants concentration, air temperature (season) and plant/support combination on the wetlands performance. Results: The Scirpus/FG combination showed the highest average removal of total COD of about 59%, and Typha/FG shows the highest removal of NH4+-N and PO4-3-P (49 and 32%, respectively). Furthermore, the removal of organic matter was independent of influent concentration, while mildly dependent of the season, unlike nutrients removal that was dependent on these two parameters. Media, plant and the plant/media combination influenced positively organic matter, ammonia and phosphorous removal, respectively. Conclusions: Overall, the results demonstrate the potential of wetlands in treatment of wastewater in Mediterranean regions and show how these can help to improve the quality of water in domestic zones without high-throughput technologies.

Analysis of manganese-regulated gene expression in the ligninolytic basidiomycete Ceriporiopsis subvermispora.

In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to identify transcript-derived fragments (TDFs), a total of 37 differentially expressed cDNA fragments were identified by comparing band intensities among cDNA-AFLP patterns obtained from mycelia from cultures supplemented with different concentrations of Mn(2+). Of 21 differentially expressed TDFs, nine were classified as upregulated, five as downregulated and seven as unregulated. Of these, six upregulated and two downregulated TDFs were selected for further characterization. The expected TDFs for the known Mn peroxidases were not isolated, but several genes encoding proteins related to protein sorting, storage and excretion of excess Mn(2+) were identified. Transcripts induced under Mn(2+) supplementation exhibited homologies to the elongation factor eEF3, a HDEL sequence binding protein and the ARD1 subunit of the N-acetyltransferase complex, among others. Overall, the results obtained in this study suggest a complex picture of Mn(2+) homeostasis and provide the possibility to search for common regulatory elements in the promoters of the novel putatively identified genes.